Kanalas J J, Makker S P
Department of Pediatrics, University of Texas Health Science Center, San Antonio 78284-7813.
J Am Soc Nephrol. 1990 Nov;1(5):792-8. doi: 10.1681/ASN.V15792.
Because of the similarities between Heymann nephritis of rat and human membranous glomerulonephritis, we searched for a protein in human kidney similar to that of the putative autoantigen of Heymann nephritis, gp330. Human gp330 was isolated by a purification scheme identical to that used for the isolation of rat gp330. The isolated human gp330 was compared with rat gp330 by peptide mapping and was found to be very similar in structure. Studies of immunologic reactivity of the rat antigen-induced Heymann nephritis nephritogenic autoantibody to human gp330 indicated that some of the rat antigen epitopes were shared with its human counterpart. The human gp330 was capable of eliciting on active Heymann nephritis response in rats when used as an immunogen. The nephritogenic autoantibody eluted from these rat kidney glomeruli showed equal binding to both rat and human antigens. Finally, a polyclonal antibody to human gp330 was able to induce passive Heymann nephritis in rats. These results demonstrate that there is a gp330 protein in human kidney that is very similar to the putative autoantigen of Heymann nephritis.
由于大鼠的海曼肾炎与人类膜性肾小球肾炎存在相似性，我们在人肾中寻找一种与海曼肾炎假定自身抗原gp330相似的蛋白质。人gp330是通过与用于分离大鼠gp330相同的纯化方案分离得到的。通过肽图谱分析将分离出的人gp330与大鼠gp330进行比较，发现其结构非常相似。对大鼠抗原诱导的海曼肾炎致肾炎自身抗体与人gp330的免疫反应性研究表明，大鼠抗原的一些表位与其人类对应物相同。当用作免疫原时，人gp330能够在大鼠中引发活动性海曼肾炎反应。从这些大鼠肾小球洗脱的致肾炎自身抗体对大鼠和人类抗原均表现出同等的结合能力。最后，一种针对人gp330的多克隆抗体能够在大鼠中诱导被动性海曼肾炎。这些结果表明，人肾中存在一种与海曼肾炎假定自身抗原非常相似的gp330蛋白质。
