Department of Plastic and Hand Surgery, Burn Center, BG-University Hospital Bergmannsheil, Ruhr-University Bochum, Bochum, Germany.
Burns. 2011 Jun;37(4):656-64. doi: 10.1016/j.burns.2011.01.004. Epub 2011 Feb 22.
The treatment of burns remains a challenge. Besides the administration of physiological saline, local disinfection and symptomatic medications, no causal therapy is known to accelerate angiogenesis and wound healing. The aim of this study was to investigate the influences of dilatative and anti-inflammatory acting drugs on microcirculation, angiogenesis and leukocyte behavior, which had shown positive effects in former burn studies.
The ears of male hairless mice (n=47) were inflicted with full thickness burns using a hot air jet. Then the affects of five intraperitoneal injections of either acetylsalicylic acid (ASA), isosorbide dinitrate, prostaglandin E1 (PGE1) or sodium chloride (each administered to one of four corresponding study groups), on microcirculation, leukocyte-endothelial interaction and angiogenesis were investigated over a 12 day period using intravital fluorescent microscopy.
Angiogenesis was slightly improved by PGE1 (0.3 vs. 1.3% non-perfused area in other groups on day 12, p=0.029). Additionally, blood flow increased and rolling leukocytes decreased compared to other groups. The ASA-group showed best functional vessel density and lowest leukocyte-adhesion. The often described posttraumatic expansion of tissue damage could not be observed in either group.
Prostaglandin E1 improved angiogenesis, increased the blood flow and reduced the number of rolling leukocytes. ASA had positive influences on functional vessel density, edema formation and the number of sticking leukocytes. However, it reduced the blood flow. Overall, out of all the drugs tested, prostaglandin seems to have the greatest positive impact on microcirculation and angiogenesis in burns.
烧伤的治疗仍然是一个挑战。除了生理盐水的应用、局部消毒和对症药物治疗外，目前还没有已知的因果治疗方法可以加速血管生成和伤口愈合。本研究旨在探讨扩张和抗炎作用药物对微循环、血管生成和白细胞行为的影响，这些药物在以前的烧伤研究中显示出积极的效果。
雄性无毛小鼠（n=47）的耳朵使用热空气射流造成全层烧伤。然后，通过活体荧光显微镜观察，研究了 5 次腹腔注射乙酰水杨酸（ASA）、异山梨酯二硝酸盐、前列腺素 E1（PGE1）或氯化钠（分别给予 4 个对应研究组中的一个）对微循环、白细胞-内皮相互作用和血管生成的影响，为期 12 天。
与其他组相比，PGE1 组的血管生成略有改善（第 12 天非灌注区为 0.3%，其他组为 1.3%，p=0.029）。此外，与其他组相比，血流增加，滚动白细胞减少。ASA 组显示出最佳的功能血管密度和最低的白细胞黏附。在任何一组中都没有观察到经常描述的创伤后组织损伤扩大。
前列腺素 E1 改善了血管生成，增加了血流量，减少了滚动白细胞的数量。ASA 对功能血管密度、水肿形成和黏附白细胞的数量有积极影响。然而，它降低了血流量。总体而言，在所测试的所有药物中，前列腺素似乎对烧伤的微循环和血管生成有最大的积极影响。
