Kanematsu N, Shibata K I
2nd Department of Oral Surgery, Asahi University School of Dentistry.
Gifu Shika Gakkai Zasshi. 1990 Dec;17(2):592-7.
Eight endodontic agents were tested for their reactivity with DNA by the rec-assay system. Employing 2 strains of Bacillus subtilis with different recombination capacities, the assay suggested that DNA damage is produced by agents for root canal cleaning containing sodium hypochloride. Other endodontic drugs, including disinfectants for caries cavities, sedatives for pulp, root canal disinfectants, and pulp devitalizing agents containing phenol, camphor, tricresol, formalin, and paraformaldehyde were also positive by rec-assay and would seem to potentially of damage cellular DNA in Bacillus subtilis. Though no clear evidence exists that drugs showing positive results by rec-assay induce malignant tumors in the oral region, these agents should be used carefully since they would seem to possibly cause genetic toxicity in mammalian cells.
通过rec检测系统对8种牙髓病治疗剂与DNA的反应性进行了测试。使用两种具有不同重组能力的枯草芽孢杆菌菌株，该检测表明，含次氯酸钠的根管清理剂会造成DNA损伤。其他牙髓病治疗药物，包括龋洞消毒剂、牙髓镇静剂、根管消毒剂以及含有苯酚、樟脑、三甲酚、福尔马林和多聚甲醛的牙髓失活剂，通过rec检测也呈阳性，似乎有可能损伤枯草芽孢杆菌的细胞DNA。尽管尚无明确证据表明rec检测呈阳性的药物会诱发口腔区域的恶性肿瘤，但这些药物似乎可能对哺乳动物细胞产生遗传毒性，因此应谨慎使用。
