Du Hui, Liu Haiyi, Zhao Jun, Wu Yuanyuan, Gong Xun, Zhou Qiong, Xu Jingjing, Li Yuqi, Shi Xinwei, Qiao Fuyuan
Department of Gynaecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
J Huazhong Univ Sci Technolog Med Sci. 2011 Feb;31(1):17-20. doi: 10.1007/s11596-011-0143-7. Epub 2011 Feb 19.
The mechanism of injury on the human glomerular endothelial cells (ciGENC) induced by preeclampsia serum was investigated. Concentration of maternal serum sFlt-1 protein was detected by ELISA. Fluorescently-labeled bovine serum albumin infiltrating through lower chamber of Transwell was measured by multifunction microplate reader. Morphologic change of ciGENC was observed under inverted phase contrast microscope. The concentration of sflt-1 in preeclampsia groups was significantly increased as compared with control group (P<0.01). Permeability in preeclampsia groups was significantly increased as compared with control group (P<0.01). By contrast with severe preeclampsia group, the permeability of ciGENC monolayer in mild preeclampsia group was decreased significantly (P<0.05). Intervention of exogenous VEGF significantly decreased permeability of ciGENC in preeclampsia groups. It was concluded that sFlt-1 increased ciGENC permeability by damaging integrity of endothelial barrier function.
研究了子痫前期血清诱导人肾小球内皮细胞（ciGENC）损伤的机制。采用酶联免疫吸附测定法（ELISA）检测母体血清可溶性血管内皮生长因子受体-1（sFlt-1）蛋白浓度。用多功能微孔板读数仪测量经Transwell小室下层渗透的荧光标记牛血清白蛋白。在倒置相差显微镜下观察ciGENC的形态变化。子痫前期组sFlt-1浓度与对照组相比显著升高（P<0.01）。子痫前期组的通透性与对照组相比显著增加（P<0.01）。与重度子痫前期组相比，轻度子痫前期组ciGENC单层的通透性显著降低（P<0.05）。外源性血管内皮生长因子（VEGF）干预显著降低子痫前期组ciGENC的通透性。得出结论：sFlt-1通过破坏内皮屏障功能的完整性增加ciGENC的通透性。
