Yongsheng Jia, Xiaoyun Ma, Xiaoli Wei, Xin Li, Haitao Yan, Xiaoyan Liu, Jianquan Zheng
Beijing Institute of Pharmacology and Toxicology, Beijing, 100850, China.
J Huazhong Univ Sci Technolog Med Sci. 2011 Feb;31(1):33-38. doi: 10.1007/s11596-011-0146-4. Epub 2011 Feb 19.
Antitumor effects of erythromycin and the related mechanism were investigated in the present study. Neuroblastoma cells (SH-SY5Y) were exposed to erythromycin at different concentrations for different durations. Cell proliferation was measured by cell counting, and cell viability was examined by MTT assay. Cell cycle phase distribution and the cytosolic calcium level were detected by flow cytometry. Mitochondrial membrane potential was measured by the JC-1 probe staining and fluorescent microscopy. The expression of an oncogene (c-Myc) and a tumor suppressor [p21 (WAF1/Cip1)] proteins was analyzed by using Western blotting. Erythromycin could inhibit the proliferation of SH-SY5Y cells in a concentration- and time-dependent manner. The cell cycle was arrested at S phase. Mitochondrial membrane potential collapsed and the cytosolic calcium was overloaded in SH-SY5Y cells when treated with erythromycin. The expression of c-Myc protein was down-regulated, while that of p21 (WAF1/Cip1) protein was up-regulated. It was concluded that erythromycin could restrain the proliferation of SH-SY5Y cells. The antitumor mechanism of erythromycin might involve regulating the expression of c-Myc and p21 (WAF1/Cip1) proteins.
本研究探讨了红霉素的抗肿瘤作用及其相关机制。将神经母细胞瘤细胞（SH-SY5Y）暴露于不同浓度的红霉素中不同时间。通过细胞计数测量细胞增殖，并通过MTT法检测细胞活力。通过流式细胞术检测细胞周期阶段分布和胞质钙水平。用JC-1探针染色和荧光显微镜测量线粒体膜电位。使用蛋白质印迹法分析癌基因（c-Myc）和肿瘤抑制因子[p21（WAF1/Cip1）]蛋白的表达。红霉素可浓度和时间依赖性地抑制SH-SY5Y细胞的增殖。细胞周期停滞于S期。用红霉素处理后，SH-SY5Y细胞的线粒体膜电位崩溃，胞质钙超载。c-Myc蛋白表达下调，而p21（WAF1/Cip1）蛋白表达上调。得出结论，红霉素可抑制SH-SY5Y细胞的增殖。红霉素的抗肿瘤机制可能涉及调节c-Myc和p21（WAF1/Cip1）蛋白的表达。
