Institute for Research in Biomedicine, IQAC-CSIC, CIBER-BBN Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Edifici Helix, Baldiri Reixac 10, Barcelona, Spain.
Chem Biodivers. 2011 Feb;8(2):287-99. doi: 10.1002/cbdv.201000274.
The synthesis of RNA molecules carrying lipids at their 3'-termini and 5'-termini is reported. These conjugates were fully characterized by MALDI-TOF mass spectrometry and HPLC chromatography. The ability of these conjugates to silence gene expression was evaluated in the inhibition of the tumor necrosis factor. All the lipid-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some lipid-siRNA derivatives can exert a slight reduction of gene expression.
报道了在 RNA 分子的 3'-末端和 5'-末端携带脂质的合成。这些缀合物通过 MALDI-TOF 质谱和 HPLC 色谱法进行了全面表征。通过肿瘤坏死因子的抑制评估了这些缀合物沉默基因表达的能力。如果用寡核苷酸转染，所有的脂质-siRNA 衍生物都与 RNA 干扰机制兼容。在没有转染试剂的情况下，一些脂质-siRNA 衍生物可以轻微降低基因表达。
