Department of Chemistry, Research Institute for Natural Sciences, Korea University, 1-Anamdong, Seoul 136-701, Korea.
Chem Asian J. 2011 May 2;6(5):1234-40. doi: 10.1002/asia.201000720. Epub 2011 Feb 17.
A series of Zn(2+)-selective two-photon fluorescent probes (AZnM1-AZnN) that had a wide range of dissociation constants (K(d) (TP) =8 nm-12 μM) were synthesized. These probes showed appreciable water solubility (>3 μM), cell permeability, high photostability, pH insensitivity at pH>7, significant two-photon action cross-sections (86-110 GM) upon complexation with Zn(2+), and can detect the Zn(2+) ions in HeLa cells and in living tissue slices of rat hippocampal at a depth of >80 μm without mistargeting and photobleaching problems. These probes can potentially find application in the detection of various amounts of Zn(2+) ions in live cells and intact tissues.
我们合成了一系列具有广泛离解常数（Kd(TP) =8 nm-12 μM）的锌（Ⅱ）选择性双光子荧光探针（AZnM1-AZnN）。这些探针具有较好的水溶性（>3 μM）、细胞通透性、高光稳定性、在 pH>7 时对 pH 不敏感、与锌（Ⅱ）配位后具有显著的双光子作用截面（86-110 GM），并且可以在不出现误靶和光漂白问题的情况下，在 >80 μm 的深度检测 HeLa 细胞和大鼠海马组织切片中的锌（Ⅱ）离子。这些探针有望应用于活细胞和完整组织中各种锌（Ⅱ）离子含量的检测。
