Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, China.
Anal Chim Acta. 2011 Aug 12;699(2):198-205. doi: 10.1016/j.aca.2011.05.015. Epub 2011 May 17.
A novel two-photon fluorescence probe for Pb(2+) derived from 4-methyl-2,5-dicyano-4'-amino stilbene as a two-photon fluorophore and bis[2-(2-aminophenylsulfanyls)ethyl]amine as a novel Pb(2+) ligand was developed. The probe possesses small molecule size, large two-photon absorption cross-section (1020 GM), noncytotoxic effect, long-wavelength emission at 609 nm, large Stokes shift (209 nm), excellent photostability, moderate water-solubility, good cell-permeability, and pH-insensitivity in the biologically relevant pH range. The probe can selectively detect Pb(2+) ions in live cells and living tissues without interference from other metal ions and the membrane-bound probes, and its quenching constant (K(SV)(TP)) is 7.58×10(5) M(-1).
一种基于 4-甲基-2,5-二氰基-4'-氨基二苯乙烯作为双光子荧光团和双[2-(2-氨苯基硫代)乙基]胺作为新型 Pb(2+)配体的新型 Pb(2+)荧光探针。该探针具有小分子尺寸、大双光子吸收截面（1020 GM）、非细胞毒性效应、609nm 处的长波长发射、大斯托克斯位移（209nm）、优异的光稳定性、适度的水溶性、良好的细胞通透性以及在生物学相关 pH 值范围内对 pH 值不敏感。该探针可选择性地检测活细胞和活组织中的 Pb(2+)离子，而不受其他金属离子和膜结合探针的干扰，其猝灭常数（K(SV)(TP)）为 7.58×10(5) M(-1)。
