Biophysics Department, Institute of Biology, University of Stuttgart, Pfaffenwaldring 57, D-70550 Stuttgart, Germany.
J Phys Condens Matter. 2010 Nov 17;22(45):454132. doi: 10.1088/0953-8984/22/45/454132. Epub 2010 Oct 29.
In the present work we applied a novel mass spectrometry method termed laser-induced liquid bead ion desorption mass spectrometry (LILBID-MS) to the outer mitochondrial membrane protein translocon TOM to analyze its subunit composition and stoichiometry. With TOM core complex, purified at high pH, we demonstrate that a TOM core complex of Neurospora crassa is composed of at least two Tom40 and Tom22 molecules, respectively, and more than five small Tom subunits between 5.5 and 6.4 kDa. We show that the multiprotein complex has a total molecular mass higher than 170 depending on the number of Tom5, Tom6 and Tom7 molecules bound.
在本工作中，我们应用了一种新型的质谱方法，称为激光诱导液珠离子解吸质谱（LILBID-MS），用于分析外膜蛋白转位通道 TOM 的亚基组成和化学计量。使用在高 pH 值下纯化的 TOM 核心复合物，我们证明了粗糙脉孢菌的 TOM 核心复合物至少由两个 Tom40 和 Tom22 分子组成，并且在 5.5 至 6.4 kDa 之间有超过五个小 Tom 亚基。我们表明，该多蛋白复合物的总分子量高于 170，具体取决于结合的 Tom5、Tom6 和 Tom7 分子的数量。
