Li Jie, Zhang Yao-Bin
College of Physical Education, Northwest Normal University, Lanzhou 730070, China.
Sheng Li Xue Bao. 2011 Feb 25;63(1):55-61.
The present study was aimed to investigate the effect of hypoxic training on mitochondrial antioxidants and activities of respiratory chain complexes in mitochondria of skeletal muscle in rats. Forty healthy male Wistar rats were randomized to 5 groups (n=8): living low-training low (LoLo), living high-training high (HiHi), living high-training low (HiLo), living low-training high (LoHi), and living high-exercise high-training low (HiHiLo). All the animals were subjected to 5-week training in normoxic (atmospheric pressure=632 mmHg, altitude of about 1 500 m) or hypoxic environment (atmospheric pressure=493 mmHg, simulated altitude of about 3 500 m). Before exhaustive running, the animals stayed in normoxia for 3 d. Skeletal muscles were prepared immediately after exhaustive running. Muscle mitochondria were extracted by differential centrifugation. Spectrophotometric analysis was used to evaluate activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), malondialdehyde (MDA) level and respiratory chain complex (C) I-III activities in muscle homogenate and mitochondria. Results showed that SOD, GSH-Px, CAT activities and MDA level in skeletal muscle homogenate in HiHi and HiHiLo groups were significantly increased (P<0.05 or P<0.01) compared with those in LoLo group. Muscle mitochondrial MDA level in HiHi and HiHiLo groups was significantly lower (P<0.01), while activities of SOD, GSH-Px and CAT were remarkably higher (P<0.01) than those in LoLo group. Meanwhile, C I-III activities in HiHi and HiHiLo groups were increased significantly (P<0.01), and C II activity in HiLo group also was increased remarkably (P<0.01) compared with those in LoLo group. These results suggest that HiHiLo might be an ideal hypoxic training mode.
本研究旨在探讨低氧训练对大鼠骨骼肌线粒体抗氧化剂及呼吸链复合体活性的影响。40只健康雄性Wistar大鼠随机分为5组（n = 8）：低住低训（LoLo）组、高住高训（HiHi）组、高住低训（HiLo）组、低住高训（LoHi）组和高住高练低训（HiHiLo）组。所有动物在常氧（大气压 = 632 mmHg，海拔约1500米）或低氧环境（大气压 = 493 mmHg，模拟海拔约3500米）下进行为期5周的训练。在力竭跑之前，动物在常氧环境中停留3天。力竭跑后立即制备骨骼肌。通过差速离心法提取肌肉线粒体。采用分光光度法分析肌肉匀浆和线粒体中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）的活性、丙二醛（MDA）水平以及呼吸链复合体（C）I-III的活性。结果显示，与LoLo组相比，HiHi组和HiHiLo组骨骼肌匀浆中SOD、GSH-Px、CAT活性及MDA水平显著升高（P < 0.05或P < 0.01）。HiHi组和HiHiLo组肌肉线粒体MDA水平显著降低（P < 0.01），而SOD、GSH-Px和CAT活性显著高于LoLo组（P < 0.01）。同时，与LoLo组相比，HiHi组和HiHiLo组C I-III活性显著增加（P < 0.01），HiLo组C II活性也显著增加（P < 0.01）。这些结果表明，HiHiLo可能是一种理想的低氧训练模式。
