Guan X Y, Trent J M
Methods Mol Med. 2001;39:247-52. doi: 10.1385/1-59259-071-3:247.
Chromosome microdissection is a recently developed molecular cytogenetic technique that has become increasingly important as a bridge connecting cytogenetics to molecular genetics. After a decade of effort, this approach has been developed into a useful and reproducible approach for several purposes, including 1) the isolation of DNA from any cytogenetically recognizable region which can be used to generate DNA microclone libraries for molecular analysis and positional cloning (1,2) the generation of fluorescence in situ hybridization (FISH) probes for whole chromosome painting probes (3), and chromosome arm painting probes (4) for cytogenetic study; 3) combined with FISH, microdissection has been applied to detecte virtually any kind of visible chromosome rearrangements (5,6); and more recently, 4) microdissection combined with hybrid selection has been applied to identify genes associated with homogeneously staining regions (HSRs) in human cancers (7,8).
染色体显微切割是一项最近发展起来的分子细胞遗传学技术，作为连接细胞遗传学和分子遗传学的桥梁，其重要性日益凸显。经过十年的努力，这种方法已发展成为一种有用且可重复的方法，可用于多种目的，包括：1）从任何细胞遗传学可识别区域分离DNA，用于构建DNA微克隆文库进行分子分析和定位克隆（1,2）；2）生成用于全染色体涂染探针（3）和染色体臂涂染探针（4）的荧光原位杂交（FISH）探针，用于细胞遗传学研究；3）与FISH相结合，显微切割已被应用于检测几乎任何类型的可见染色体重排（5,6）；最近，4）显微切割与杂交选择相结合已被用于鉴定人类癌症中与均匀染色区（HSR）相关的基因（7,8）。
