Perry D J
Department of Haematology, Haemophilia Centre and Haemostasis Unit, Royal Free Hospital School of Medicine, London, UK.
Methods Mol Med. 1999;31:31-8. doi: 10.1385/1-59259-248-1:31.
The polymerase chain reaction (PCR) has revolutionized many areas of medicine, including hemostasis. Although this volume is not devoted to PCR, many of the chapters employ the technique at some point to amplify specific DNA or RNA sequences. This chapter, therefore, provides a brief outline of the techniques and methods for the amplification of both DNA and RNA. It should be remembered that no single protocol will be suitable for all PCR reactions and that optimization of the reaction is necessary whenever a new PCR reaction is undertaken. Further information on PCR can be found in one of the many textbooks on the subject that are available.
聚合酶链反应（PCR）已经彻底改变了医学的许多领域，包括止血领域。尽管本书并非专门论述PCR，但许多章节在某些时候会采用该技术来扩增特定的DNA或RNA序列。因此，本章简要概述了DNA和RNA扩增的技术和方法。应该记住，没有一种单一的方案适用于所有的PCR反应，并且每当进行新的PCR反应时，对反应进行优化都是必要的。关于PCR的更多信息可以在众多关于该主题的教科书中找到。
