Takahashi T, Takahashi K, Daniel T O
Department of Cell Biology, Vanderbilt University, Nashville, TN.
Methods Mol Med. 1999;30:307-14. doi: 10.1385/1-59259-247-3:307.
Cultured endothelial cells have provided a powerful tool for discovery of the molecular regulators of a range of vascular processes from angiogenesis to fibrinolysis (1). Yet, the utility of genetic manipulation of endothelial culture systems to dissect critical intracellular signaling processes has been limited to date. Available methods such as retroviral transduction require endothelial proliferation, while cationic lipid mediated transfection is inefficient and evokes marked toxicity in cultured endothelial cells (2-4). Adenoviral transduction of endothelial cells is efficient, but preparation of recombinant adenovirus vectors is cumbersome.
培养的内皮细胞为发现从血管生成到纤维蛋白溶解等一系列血管过程的分子调节因子提供了一个强大的工具(1)。然而，迄今为止，利用内皮细胞培养系统进行基因操作来剖析关键的细胞内信号传导过程的效用有限。诸如逆转录病毒转导等现有方法需要内皮细胞增殖，而阳离子脂质介导的转染效率低下，并且在培养的内皮细胞中会引发明显的毒性(2 - 4)。内皮细胞的腺病毒转导效率很高，但重组腺病毒载体的制备很繁琐。
