Sindhu Edakkadath R, Preethi Korengath C, Kuttan Ramadasan
Amala Cancer Research Centre, Amala Nagar, Thrissur 680 555, India.
Indian J Exp Biol. 2010 Aug;48(8):843-8.
Carotenoid lutein was evaluated for its antioxidant potential both in vitro and in vivo. Lutein was found to scavenge superoxide radicals, hydroxyl radicals and inhibited in vitro lipid peroxidation. Concentrations needed for 50% inhibition (IC50) were 21, 1.75 and 2.2 microg/mL respectively. It scavenged 2,2-diphenyl-1-picryl hydrazyl (IC50 35 microg/mL) and nitric oxide radicals (IC50 3.8 microg/mL) while 2,2-azobis-3-ethylbenzthiozoline-6-sulfonic acid radicals were inhibited at higher concentration. Ferric reducing power (50%) of lutein was found to be equal 0.3 micromols/mL of FeSO4.7H2O. Its oral administration inhibited superoxide generation in macrophages in vivo by 34.18, 64.32 and 70.22% at doses of 50, 100 and 250 mg/kg body weight. The oral administration of lutein in mice for 1 month significantly increased the activity of catalase, superoxide dismutase, glutathione reductase and glutathione in blood and liver while the activity of glutathione peroxidase and glutathione-S-transferase were found to be increased in the liver tissue. Implication of these results in terms of its role in reducing degenerative diseases is discussed.
对类胡萝卜素叶黄素的体外和体内抗氧化潜力进行了评估。发现叶黄素可清除超氧自由基、羟基自由基，并抑制体外脂质过氧化。50%抑制率（IC50）所需的浓度分别为21、1.75和2.2微克/毫升。它可清除2,2-二苯基-1-苦基肼（IC50为35微克/毫升）和一氧化氮自由基（IC50为3.8微克/毫升），而在较高浓度下可抑制2,2-偶氮二（3-乙基苯并噻唑啉-6-磺酸）自由基。发现叶黄素的铁还原能力（50%）相当于0.3微摩尔/毫升的FeSO4·7H2O。以50、100和250毫克/千克体重的剂量口服叶黄素可在体内抑制巨噬细胞中超氧的产生，抑制率分别为34.18%、64.32%和70.22%。在小鼠中口服叶黄素1个月可显著提高血液和肝脏中过氧化氢酶、超氧化物歧化酶、谷胱甘肽还原酶和谷胱甘肽的活性，而肝脏组织中谷胱甘肽过氧化物酶和谷胱甘肽-S-转移酶的活性则有所增加。讨论了这些结果在其减少退行性疾病方面的作用。
