Cockburn A F, Mitchell S E, Seawright J A
Insects Affecting Man and Animals Research Laboratory, USDA, ARS, Gainesville, Florida.
Arch Insect Biochem Physiol. 1990;14(1):31-6. doi: 10.1002/arch.940140104.
The entire 15 kilobase (kb) Anopheles quadrimaculatus mitochondrial DNA (mtDNA) was cloned as three EcoRI fragments in a bacteriophage vector and then subcloned into plasmid vectors. The cloned DNA was physically mapped with restriction endonucleases, and the maps were compared to the restriction patterns of native A. quadrimaculatus mtDNA. Several genes were mapped by sequencing the ends of A. quadrimaculatus mtDNA subclones and by hybridization with the previously characterized Aedes albopictus mtDNA clones. These portions of the genetic map were identical in gene order to those of Drosophila yakuba. The predicted amino acid sequence of the protein coding regions that were sequenced were between 72% and 98% homologous to D. yakuba. The cloned mtDNA will be useful as a probe for population genetic analysis of mosquitoes.
将15千碱基（kb）的四斑按蚊线粒体DNA（mtDNA）作为三个EcoRI片段克隆到噬菌体载体中，然后亚克隆到质粒载体中。用限制性内切酶对克隆的DNA进行物理图谱绘制，并将图谱与天然四斑按蚊mtDNA的限制性图谱进行比较。通过对四斑按蚊mtDNA亚克隆末端进行测序以及与先前鉴定的白纹伊蚊mtDNA克隆进行杂交，对几个基因进行了定位。遗传图谱的这些部分在基因顺序上与雅库布果蝇的相同。测序的蛋白质编码区预测氨基酸序列与雅库布果蝇的同源性在72%至98%之间。克隆的mtDNA将作为蚊虫群体遗传分析的探针。
