Naylor S, Gibbs A H, Lamb J H, De Matteis F
MRC Toxicology Unit, Carshalton, Surrey, UK.
Rapid Commun Mass Spectrom. 1990 Oct;4(10):406-9. doi: 10.1002/rcm.1290041013.
A low-energy (5-450 eV) collision-activated dissociation (CAD) study of a series of aliphatic amines revealed that at collision energies above 200 eV, charge-site-initiated fragmentation occurs. The resulting fragment ions can be utilized in the characterization of alkyl substituents of di- and trisubstituted aliphatic amines. In the presence of Cu2+ and a suitable nucleophile, such as n-dodecylamine, N-alkyl protoporphyrins dealkylate to afford copper protoporphyrin and an alkyl-dodecylamine adduct. A CAD study of a number of alkyl-dodecylamine adducts derived from the copper-induced dealkylation of synthetic N-alkyl protoporphyrins, using charge-site-initiated fragment ions, showed that the alkyl group was trapped by the nucleophilic amine present. Subsequently this method was used to identify the alkyl group of a biologically derived N-alkyl protoporphyrin.
对一系列脂肪胺进行的低能量（5 - 450 eV）碰撞激活解离（CAD）研究表明，在碰撞能量高于200 eV时，会发生电荷位点引发的碎片化。产生的碎片离子可用于表征二取代和三取代脂肪胺的烷基取代基。在Cu2+和合适的亲核试剂（如正十二胺）存在下，N - 烷基原卟啉会发生脱烷基反应，生成铜原卟啉和烷基 - 十二胺加合物。使用电荷位点引发的碎片离子，对源自合成N - 烷基原卟啉铜诱导脱烷基反应的多种烷基 - 十二胺加合物进行的CAD研究表明，烷基被存在的亲核胺捕获。随后，该方法被用于鉴定生物来源的N - 烷基原卟啉的烷基。
