School of Biological Sciences, University of Bristol, Bristol, United Kingdom.
Zebrafish. 2011 Mar;8(1):17-22. doi: 10.1089/zeb.2010.0671. Epub 2011 Feb 22.
Zebrafish have been shown to have preference for light or dark environments depending on the ambient light level and the presence or absence of food odor. We used a cylindrical tank, half of which was surrounded by a black surface and the other half by white, to elicit a choice from individual wild-type, adult zebrafish. One treatment group was exposed to food odor and the other to water (as a control) at the beginning of the trial. During 10-min trials, the light level was increased each minute over a fivefold range in steps from 1.34 × 10(17) photons/s/m(2) at the beginning to a final light level of 8.31 × 10(17) photons/s/m(2). We demonstrate that the preference of the zebrafish for the light or dark half of the cylinder is dependent upon ambient light levels as well as olfactory stimulation. These results provide a potential explanation for the contradictory observations that, when given a choice, adult zebrafish prefer brighter light environments (Gerlai et al., 2000) or darker light environments (Serra et al., 1999). Thus, we present data useful in designing more powerful and reliable behavioral assays for use with zebrafish as well as further information about the effect of olfactory stimulation on zebrafish visual behavior.
斑马鱼会根据周围环境的光照水平以及食物气味的存在与否，表现出对光环境或暗环境的偏好。我们使用一个圆柱形水箱，其一半被黑色表面包围，另一半被白色包围，以此来让野生型成年斑马鱼做出选择。一组处理组在试验开始时暴露于食物气味中，另一组则暴露于水中（作为对照）。在 10 分钟的试验中，光照水平在 5 倍范围内以每分钟增加 1 倍的方式从 1.34×10(17) 光子/s/m(2)逐渐增加到最终的 8.31×10(17) 光子/s/m(2)。我们证明了斑马鱼对圆柱状鱼缸亮面或暗面的偏好取决于周围的光照水平和嗅觉刺激。这些结果为成年斑马鱼在有选择的情况下更喜欢明亮的光照环境（Gerlai 等人，2000）或更喜欢黑暗的光照环境（Serra 等人，1999）这两种相互矛盾的观察结果提供了一种可能的解释。因此，我们提供了有用的数据，可以用于设计更强大、更可靠的斑马鱼行为测定法，同时也提供了关于嗅觉刺激对斑马鱼视觉行为影响的进一步信息。
