Department of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Goiânia, Brazil.
J Oral Pathol Med. 2011 May;40(5):380-4. doi: 10.1111/j.1600-0714.2011.01014.x. Epub 2011 Feb 22.
Perforin and granzyme B (GB) are the main constituents of cytotoxic T-lymphocyte granules, and they have important roles in preventing the initiation and progression of cancer.
The aim of this study was to compare the expression of CD8(+) /perforin(+) double-staining and GB(+) cells, by immunohistochemistry, in primary oral cavity squamous cell carcinoma (OCSCC), lip squamous cell carcinoma (LSCC), non-dysplastic leukoplakia (LK), dysplastic LK, actinic cheilitis (AC), oral lichen planus (LP) and normal oral mucosa.
Our results showed a higher expression of CD8(+) /perforin(+) and GB(+) cells in LSCC when compared with the samples of OCSCC, non-dysplastic and dysplastic LK, AC, oral LP and normal oral mucosa. In addition, increased CD8(+) /perforin(+) and GB(+) cell numbers were observed in all pre-malignant lesions (non-dysplastic LK, dysplastic LK, AC) when compared with the control.
Perforin and GB proteins may contribute to antitumoural immunity, leading to the direct killing of tumour cells; however, it seems to occur more effectively in LSCC than OCSCC.
穿孔素和颗粒酶 B（GB）是细胞毒性 T 淋巴细胞颗粒的主要成分，它们在预防癌症的发生和发展中起着重要作用。
本研究旨在通过免疫组织化学比较原发性口腔鳞状细胞癌（OCSCC）、唇鳞状细胞癌（LSCC）、非异型增生性白色角化病（LK）、异型增生性 LK、光化性唇炎（AC）、口腔扁平苔藓（LP）和正常口腔黏膜中 CD8（+）/穿孔素（+）双染色和 GB（+）细胞的表达。
我们的结果表明，LSCC 中 CD8（+）/穿孔素（+）和 GB（+）细胞的表达高于 OCSCC、非异型增生性和异型增生性 LK、AC、口腔 LP 和正常口腔黏膜的样本。此外，与对照组相比，所有癌前病变（非异型增生性 LK、异型增生性 LK、AC）中 CD8（+）/穿孔素（+）和 GB（+）细胞数量增加。
穿孔素和 GB 蛋白可能有助于抗肿瘤免疫，导致肿瘤细胞的直接杀伤，但在 LSCC 中似乎比在 OCSCC 中更有效。
