[非小细胞肺癌中MTAP蛋白及基因表达的检测]
[Detection of MTAP protein and gene expression in non-small cell lung cancer].
作者信息
Li Shasha, Zhang Yijun, Li Hongli, Ding Baoqing
机构信息
Department of Pharmacology, Weifang Medical College, Weifang 261053, China.
出版信息
Zhongguo Fei Ai Za Zhi. 2011 Feb;14(2):151-5. doi: 10.3779/j.issn.1009-3419.2011.02.09.
BACKGROUND AND OBJECTIVE
The abnormal expression of MTAP, a tumor suppressor gene, is found in a variety of tumor tissues. The aim of this study is to detect the expression of MTAP mRNA protein and the clinical significance for the therapy of non-small cell lung cancer tissue (NSCLC).
METHODS
The expression of MTAP protein was detected by immunohistochemistry in 52 cases of NSCLC patients. The relative expression MTAP mRNA was detected by real-time quantitative PCR.
RESULTS
The expression of MTAP protein in NSCLC tissue was significantly lower than that in paracarcinomous tissue and borderline lung tissue respectively (t=10.283, 10.940, P < 0.001). There was no significant difference among gender, age, smoking history, histology except differentiation (t=2.310, P=0.025). The MTAP mRNA relative expression in NSCLC tissue was significantly lower than that in paracarcinomous tissue (t=9.902, P < 0.001).
CONCLUSIONS
Downregulation of MTAP protein and gene expression is correlated to the oncogenesis and progression of NSCLC.
背景与目的
肿瘤抑制基因MTAP在多种肿瘤组织中存在异常表达。本研究旨在检测MTAP mRNA蛋白在非小细胞肺癌组织（NSCLC）中的表达情况及其对治疗的临床意义。
方法
采用免疫组织化学法检测52例NSCLC患者MTAP蛋白的表达。采用实时定量PCR检测MTAP mRNA的相对表达量。
结果
NSCLC组织中MTAP蛋白的表达分别显著低于癌旁组织和肺交界组织（t=10.283，10.940，P<0.001）。除分化程度外，性别、年龄、吸烟史、组织学类型之间差异无统计学意义（t=2.310，P=0.025）。NSCLC组织中MTAP mRNA的相对表达量显著低于癌旁组织（t=9.902，P<0.001）。
结论
MTAP蛋白和基因表达下调与NSCLC的发生发展相关。
Zotero 插件