[评估两种市售免疫层析法用于快速诊断人类粪便标本中的十二指肠贾第虫和隐孢子虫属]
[Assessment of two commercially available immunochromatographic assays for a rapid diagnosis of Giardia duodenalis and Cryptosporidium spp. in human fecal specimens].
作者信息
Gutiérrez-Cisneros María José, Martínez-Ruiz Rocío, Subirats Mercedes, Merino Francisco Jesús, Millán Rosario, Fuentes Isabel
机构信息
Servicio de Parasitología, Centro Nacional de Microbiología, ISCIII, Majadahonda, Madrid, España.
出版信息
Enferm Infecc Microbiol Clin. 2011 Mar;29(3):201-3. doi: 10.1016/j.eimc.2010.09.005. Epub 2011 Feb 20.
INTRODUCTION
To assess and compare the performance of two immunochromatographic tests for the simultaneous detection of Giardia duodenalis and Cryptosporidium spp. in faeces.
MATERIALS AND METHODS
In this study 254 faeces samples were tested using the two immunochromatography strips Cryto-Giardia (CerTest Biotec) and Stick Crypto-Giardia (Operon).
RESULTS
In the diagnosis of G. duodenalis, the sensitivity and specificity of the kits were 97% and 100%, respectively for the CerTest; and 97% and 95% for Operon. In the diagnosis of Cryptosporidium spp. Certest strip rendering a sensitivity of 100%, compared to with a sensitivity of 92% using Operon. There were no false positives using either technique.
CONCLUSIONS
Both methods yielded good sensitivity and specificity values and are thus useful tools for a rapid diagnosis of G. duodenalis and Cryptosporidium spp. The benefits of immunochromatography methods are that there is no requirement for expert microscopists or special equipment.
引言
评估和比较两种免疫层析检测法同时检测粪便中十二指肠贾第虫和隐孢子虫属的性能。
材料与方法
在本研究中，使用两种免疫层析试纸条，即Cryto-Giardia（CerTest Biotec公司）和Stick Crypto-Giardia（Operon公司）对254份粪便样本进行检测。
结果
在十二指肠贾第虫的诊断中，CerTest试剂盒的灵敏度和特异性分别为97%和100%；Operon试剂盒的灵敏度和特异性分别为97%和95%。在隐孢子虫属的诊断中，CerTest试纸条的灵敏度为100%，而Operon试纸条的灵敏度为92%。两种技术均未出现假阳性结果。
结论
两种方法均具有良好的灵敏度和特异性，是快速诊断十二指肠贾第虫和隐孢子虫属的有用工具。免疫层析法的优点是无需专业显微镜检查人员或特殊设备。
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