Hooshyar Hossein, Rostamkhani Parvin, Arbabi Mohsen, Delavari Mahdi
Department of Medical Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran.
Gastroenterol Hepatol Bed Bench. 2019 Winter;12(1):3-12.
Giardiasis has a global distribution and it is a common cause of diarrhea in both children and adults and is transmitted via the fecal-oral route through direct or indirect ingestion of cysts. The laboratory diagnosis of Giardia spp. is mainly based on demonstration of microscopic cyst or trophozoite in stool samples but several immunological-based assays and molecular methods are also available for giardiasis diagnosis. The aim of this study was to conduct a review of the applied methods in medical laboratory and to highlight pitfalls and challenges of them for diagnosis of giardiasis. In this article we have evaluated the Giardia diagnostic methods with a broad review of literature, electronic databases and books. The search has covered the articles and some textbooks that have published up to 2018. It has been concluded that traditional microscopy combination with stool concentration method should still be held in the routine medical laboratory due to economical and high sensitivity and immunological-based assay and molecular methods which are recommended to use as a complementary test to the traditional technique.
贾第虫病在全球范围内均有分布，是儿童和成人腹泻的常见病因，通过直接或间接摄入包囊经粪-口途径传播。贾第虫属的实验室诊断主要基于粪便样本中显微镜下包囊或滋养体的检出，但也有几种基于免疫的检测方法和分子方法可用于贾第虫病的诊断。本研究的目的是对医学实验室中应用的方法进行综述，并强调其在贾第虫病诊断中的缺陷和挑战。在本文中，我们通过广泛查阅文献、电子数据库和书籍，对贾第虫诊断方法进行了评估。检索涵盖了截至2018年发表的文章和一些教科书。得出的结论是，由于经济实惠且灵敏度高，传统显微镜检查与粪便浓缩法仍应保留在常规医学实验室中，而基于免疫的检测方法和分子方法则建议用作传统技术的补充检测。
