Magi Barbara, Migliorini Lucia
Clinic of Infectious Diseases, Department of Molecular Biology, University of Siena, Siena, Italy.
New Microbiol. 2011 Jan;34(1):93-5. Epub 2011 Jan 15.
Toxoplasmosis is a common congenital infection. It does not usually produce recognizable signs of infection at birth so most infected newborns are not detected by routine clinical examination and remain untreated. Infected children without clinical symptoms should nonetheless be identified and treated as early as possible. Serological diagnosis of congenital toxoplasmosis is quite difficult. The aim of this study was to evaluate the utility of Western blot for the diagnosis of congenital toxoplasmosis. We compared the immunological profiles of mothers and children to differentiate between passively transmitted maternal antibodies and antibodies synthesized by the infants in the first three months of life. The method enabled us to diagnose congenital toxoplasmosis in cases in which the infection had not been detected by classical serology techniques.
弓形虫病是一种常见的先天性感染。它通常在出生时不会产生可识别的感染迹象，因此大多数受感染的新生儿在常规临床检查中未被发现，也未得到治疗。然而，没有临床症状的受感染儿童仍应尽早被识别并接受治疗。先天性弓形虫病的血清学诊断相当困难。本研究的目的是评估蛋白质印迹法在先天性弓形虫病诊断中的实用性。我们比较了母亲和儿童的免疫图谱，以区分被动传递的母体抗体和婴儿在出生后前三个月合成的抗体。该方法使我们能够在经典血清学技术未检测到感染的情况下诊断先天性弓形虫病。
