Department of Pharmacology, College of Oriental Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
Amino Acids. 2012 May;42(5):1609-18. doi: 10.1007/s00726-011-0847-9. Epub 2011 Feb 23.
The increasing occurrences of allergic disorders may be attributed to exposure to environmental factors that contribute to the pathogenesis of allergy. The health benefits of green tea have been widely reported but are largely unsubstantiated. Theanine is the major amino acid present in green tea. In this study, we investigated the role of theanine in both IgE- and non- IgE-induced allergic response. Theanine inhibited compound 48/80-induced systemic anaphylactic shock and ear swelling responses. IgE-mediated passive cutaneous anaphylaxis was inhibited by the oral administration or pharmaceutical acupuncture of theanine. Histamine release from mast cells was decreased with the treatment of theanine. Theanine also repressed phorbol 12-myristate 13-acetate and calcium ionophore A23187-induced TNF-α, IL-1β, IL-6, and IL-8 secretion by suppressing NF-κB activation. Furthermore, theanine suppressed the activation of caspase-1 and the expression of receptor interacting protein-2. The current study demonstrates for the first time that theanine might possess mast cell-stabilizing capabilities.
过敏症的发生率不断增加可能归因于暴露于导致过敏发病的环境因素。绿茶的健康益处已被广泛报道，但很大程度上尚未得到证实。茶氨酸是绿茶中主要的氨基酸。在这项研究中，我们研究了茶氨酸在 IgE 和非 IgE 诱导的过敏反应中的作用。茶氨酸抑制了化合物 48/80 诱导的全身性过敏休克和耳部肿胀反应。茶氨酸的口服给药或药物针灸抑制了 IgE 介导的被动皮肤过敏反应。茶氨酸处理后，肥大细胞释放的组胺减少。茶氨酸还通过抑制 NF-κB 激活来抑制佛波醇 12-肉豆蔻酸 13-乙酸酯和钙离子载体 A23187 诱导的 TNF-α、IL-1β、IL-6 和 IL-8 分泌。此外，茶氨酸抑制了半胱天冬酶-1的激活和受体相互作用蛋白-2 的表达。本研究首次表明，茶氨酸可能具有稳定肥大细胞的能力。
