Soybean Research Institute (Key Laboratory of Soybean Biology in Chinese Ministry of Education), Northeast Agricultural University, Harbin, China.
Theor Appl Genet. 2011 May;122(8):1481-8. doi: 10.1007/s00122-011-1547-2. Epub 2011 Feb 23.
Linolenic acid (LN) in soybean (Glycine max L. Merr.) seed mainly contributes to the undesirable odors and flavors commonly associated with poor oil quality. LN deposition at various stages of soybean seed development had not been reported by 2010. The objects of this study were (1) to identify and measure quantitative trait loci (QTL) underlying LN content and (2) to estimate the QTL effects expressed from earlier seed developmental stages to drying seed of soybean. One hundred and twenty-five F(5:8) and F(5:9) recombinant inbred lines derived from the cross of soybean cultivars 'Hefeng 25' and 'Dongnong L5' were used for the identification of QTL underlying LN content from the 37 day (D) to 86D stages after flowering, at Harbin in 2008 and 2009. QTL × Environment interactions (QE) effects were evaluated using a mixed genetic model (Zhu in J Zhejiang Univ (Natural Science) 33:327-335, 1999). Twelve unconditional QTL and 12 conditional QTL associated with LN content were identified at different developmental stages. Most of the QTL explained <10% of phenotypic variation of LN content. Unconditional QTL QLNF-1, QLNC2-1, QLND1b-1, QLNA2-1 and QLNH-1 influenced LN content across different development stages and environments. Conditional QTL QLNF-1, QLNC2-1 and QLNH-1 were identified in multiple developmental stages and environments. Conditional and unconditional QTL clustered in neighboring intervals on linkage groups A2, C2 and D1b. Ten QTL with conditional additive main effects (a) and/or conditional additive × environment interaction effects (ae) at specific developmental stage were identified on nine linkage groups. Of them, six QTL only possessed additive main effects and seven QTL had significant ae effects in different developmental stages. A total of 13 epistatic pairwise QTL were identified by conditional mapping in different developmental stages. Two pairs of QTL only showed aa effects and five pairs of QTL only showed aae effects at different developmental stages. QTL with aa effects, as well as their environmental interaction effects, appeared to vary at different developmental stages.
亚油酸（LN）在大豆（Glycine max L. Merr.）种子中主要导致与油质差相关的不良气味和味道。截至 2010 年，尚未报道 LN 在大豆种子发育的各个阶段的沉积情况。本研究的目的是（1）鉴定和测量亚油酸含量的数量性状位点（QTL），（2）估计从早期种子发育阶段到干燥种子阶段表达的 QTL 效应。125 个来自大豆品种 'Hefeng 25' 和 'Dongnong L5' 的杂交后代 F(5:8) 和 F(5:9) 重组自交系被用于鉴定 2008 年和 2009 年在哈尔滨开花后 37 天（D）至 86D 阶段的亚油酸含量的 QTL。利用混合遗传模型（Zhu in J Zhejiang Univ (Natural Science) 33:327-335, 1999）评估了 QTL × 环境互作（QE）效应。在不同的发育阶段鉴定到 12 个与 LN 含量相关的无条件 QTL 和 12 个条件 QTL。大多数 QTL 解释了 LN 含量表型变异的<10%。无条件 QTL QLNF-1、QLNC2-1、QLND1b-1、QLNA2-1 和 QLNH-1 影响不同发育阶段和环境中的 LN 含量。条件 QTL QLNF-1、QLNC2-1 和 QLNH-1 在多个发育阶段和环境中被鉴定出来。条件和无条件 QTL 在连锁群 A2、C2 和 D1b 上的相邻区间聚集。在九个连锁群上，在特定发育阶段，有 10 个 QTL 具有条件加性主效应（a）和/或条件加性×环境互作效应（ae）。其中，6 个 QTL仅具有加性主效应，7 个 QTL在不同的发育阶段具有显著的 ae 效应。在不同的发育阶段通过条件作图鉴定到 13 个上位性两两 QTL。在不同的发育阶段，有两对 QTL 仅显示 aa 效应，有五对 QTL 仅显示 aae 效应。具有 aa 效应的 QTL 及其环境互作效应似乎在不同的发育阶段有所不同。
