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人皮蝇(双翅目:狂蝇科)卵巢的超微结构。I. 三龄幼虫期的发育

Ultrastructure of the ovary of Dermatobia hominis (Diptera: Cuterebridae). I. Development during the 3rd larval instar.

作者信息

Gregório E A, Secco V N, Toledo L A, Lello E

机构信息

Instituto de Biociências, UNESP, Botucatu, Brasil.

出版信息

Mem Inst Oswaldo Cruz. 1990 Jul-Sep;85(3):305-13. doi: 10.1590/s0074-02761990000300007.

DOI:10.1590/s0074-02761990000300007
PMID:2134704
Abstract

The ultrastructure and distribution of gonial and somatic cells in the ovary of Dermatobia hominis was studied during the 3rd larval instar. In larvae weighing between 400 and 500 mg, the ovary is partially divided into basal and apical regions by oblong somatic cells that penetrate from the periphery; these cells show ovoid nucleus and cytoplasm full of microtubules. In both regions, gonial cells with regular outlines, large nucleus and low electron-density cytoplasm are scattered among the interstitial somatic cells. These later cells have small nucleus and electrondense cytoplasm. Clear somatic cells with small nucleus and cytoplasm of very low electron-density are restrict to the apical region of the gonad. Degenerating interstitial somatic cells are seen in the basal portion close to the ovary peduncle. During all this larval period the morphological features of the ovary remain almost the same. At the end of the period there is a gradual deposition of glycogen in the cytoplasm of the somatic cells, increase in the number and density of their mitochondria plus nuclear modification as membrane wrinkling and chromatin condensation in masses.

摘要

在人皮蝇第三龄幼虫期,对其卵巢中的生殖细胞和体细胞的超微结构及分布进行了研究。在体重400至500毫克的幼虫中,卵巢被从周边穿透进来的长方形体细胞部分地分为基部和顶部区域;这些细胞具有卵圆形细胞核和充满微管的细胞质。在这两个区域中,轮廓规则、细胞核大且细胞质电子密度低的生殖细胞散布在间质体细胞之间。这些后者细胞细胞核小且细胞质电子密度高。细胞核小且细胞质电子密度极低的清亮体细胞局限于性腺的顶部区域。在靠近卵巢柄的基部部分可见退化的间质体细胞。在整个幼虫期,卵巢的形态特征几乎保持不变。在该时期结束时,体细胞的细胞质中逐渐有糖原沉积,其线粒体的数量和密度增加,同时细胞核发生变化,如膜起皱和染色质聚集成块。

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