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对安氏大蚊唾液腺的电子显微镜研究。

An electron microscopic study of the salivary gland of Rhynchosciara angelae.

作者信息

Araujo Jorge T C, Machado R D, de Souza W

出版信息

Cell Tissue Res. 1980;207(3):449-61. doi: 10.1007/BF00224619.

DOI:10.1007/BF00224619
PMID:7397756
Abstract

The structure of the salivary gland of the dipteran insect Rhynchosciara angelae in a defined stage of the larval development, characterized by the synthesis and storage of secretion product, is described. Observations were made with both Nomarski optics and electron microscopy. Filiform projections extending into the lumen of the gland were observed in the apical portion of the cells. At the basal region junctions, characterized as hemidesmosomes, were observed between the membrane of the cell and the basal lamina. The plasma membrane presents numerous infoldings into the cell increasing considerably the surface area at this region. Throughout the cytoplasm of the gland cells numerous mitochondria, Golgi complexes, microtubules, profiles of endoplasmic reticulum, secretion granules and glycogen granules were observed. Carbohydrates were detected on ultrathin sections by using the periodic acid-silver methenamine and the periodic acid-thiosemicarbazide-silver proteinate techniques.

摘要

描述了双翅目昆虫安吉拉巨蚊幼虫发育特定阶段唾液腺的结构,其特征为分泌产物的合成与储存。使用诺马斯基光学显微镜和电子显微镜进行了观察。在细胞顶端部分观察到延伸至腺腔的丝状突起。在细胞基部区域,观察到细胞与基膜之间的连接,其特征为半桥粒。质膜呈现出大量向细胞内的褶皱,显著增加了该区域的表面积。在腺细胞的整个细胞质中,观察到大量线粒体、高尔基体、微管、内质网轮廓、分泌颗粒和糖原颗粒。通过使用高碘酸-银甲胺和高碘酸-硫代半卡巴腙-银蛋白技术在超薄切片上检测到了碳水化合物。

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本文引用的文献

1
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.在电子显微镜检查中,将高pH值的柠檬酸铅用作电子不透明染色剂。
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[Morphology of glycogen. Electron microscopic study of the negative stains of particulate glycogen].[糖原的形态学。颗粒状糖原负染的电子显微镜研究]
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Exp Cell Res. 1970 Jun;60(3):327-37. doi: 10.1016/0014-4827(70)90525-2.
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The zeiss-Nomarski differential interference equipment for transmitted-light microscopy.用于透射光显微镜的蔡司-诺马斯基微分干涉仪设备。
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Cytoplasmic fine structure of Sciara salivary glands. I. Secretion.果蝇唾液腺的细胞质精细结构。I. 分泌
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An improved silver methenamine technique for the detection of periodic acid-reactive complex carbohydrates with the electron microscope.一种改良的六亚甲基四胺银技术,用于在电子显微镜下检测高碘酸反应性复合碳水化合物。
J Histochem Cytochem. 1967 Jul;15(7):409-12. doi: 10.1177/15.7.409.
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Ultrastructural aspects of histolytic processes in the salivary gland cells during metamorphic stages in Rhynchosciara hollaenderi (Diptera, Sciaridae).霍氏大蚊(双翅目,大蚊科)变态阶段唾液腺细胞组织溶解过程的超微结构特征
Cell Differ. 1975 Oct;4(4):219-36. doi: 10.1016/0045-6039(75)90028-7.
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Microtubules and thyroid hormone mobilization.微管与甲状腺激素的动员
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