Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou 215006, People's Republic of China.
J Mol Neurosci. 2011 Oct;45(2):145-53. doi: 10.1007/s12031-011-9504-8. Epub 2011 Feb 24.
Foxj1 is a member of the Forkhead/winged-helix (Fox) family of transcription factors, which is required for postnatal differentiation of ependymal cells and a subset of astrocytes in the subventricular zone. The subpopulation of astrocytes has the ability of self-renew and neurogenic potential differentiated into astrocytes, oligodendrocytes, and neurons. However, its expression and function in the central nervous system lesion are not well understood. In this study, we performed a traumatic brain injury (TBI) model in adult rats and investigated the changed expression of Foxj1 in the brain cortex. Western blot and immunohistochemistry analysis showed that the expression of Foxj1 gradually increased, reached a peak at day 3 after TBI, and declined during the following days. Double immunofluorescence staining revealed that Foxj1 was co-expressed with MAP-2 and GFAP. In addition, we detected that Ki67 had the co-localization with NeuN, GFAP, and Foxj1. All our findings suggested that Foxj1 may be involved in the pathophysiology of brain after TBI.
Foxj1 是叉头/翼状螺旋（Fox）转录因子家族的成员，对于室管膜细胞和脑室下区的一部分星形胶质细胞的出生后分化是必需的。星形胶质细胞亚群具有自我更新和神经发生潜能，可以分化为星形胶质细胞、少突胶质细胞和神经元。然而，其在中枢神经系统损伤中的表达和功能尚不清楚。在本研究中，我们在成年大鼠中进行了创伤性脑损伤（TBI）模型，并研究了 Foxj1 在大脑皮层中的表达变化。Western blot 和免疫组织化学分析表明，Foxj1 的表达逐渐增加，在 TBI 后第 3 天达到峰值，并在随后的几天内下降。双免疫荧光染色显示 Foxj1 与 MAP-2 和 GFAP 共表达。此外，我们还检测到 Ki67 与 NeuN、GFAP 和 Foxj1 共定位。所有这些发现表明，Foxj1 可能参与 TBI 后的脑病理生理学过程。
