Department of Anatomy, School of Basic Medical and Biological Sciences, Medical College of Soochow University, Soochow University, Suzhou, 215021, People's Republic of China.
J Mol Histol. 2012 Apr;43(2):195-201. doi: 10.1007/s10735-011-9383-x. Epub 2012 Apr 4.
Numblike (Numbl) plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. And Numbl is specifically expressed in the brain. However, its expression and function in the central nervous system lesion are still unclear. In this study, we performed a traumatic brain injury (TBI) model in adult rats and investigated the dynamic changes of Numbl expression in the brain cortex. Western blot and immunohistochemistry analysis revealed that Numbl was present in normal brain. It gradually decreased, reached the lowest point at day 3 after TBI, and then increased during the following days. Double immunofluorescence staining showed that Numbl immunoreactivity was found in neurons, but not astrocytes and microglia. Moreover, the 3rd day post injury was the apoptotic peak implied by the alteration of caspase-3. All these results suggested that Numbl may be involved in the pathophysiology of TBI and further research is needed to have a good understanding of its function and mechanism.
Numblike（Numbl）在室管膜壁完整性和侧脑室神经母细胞存活中发挥重要作用。Numbl 特异性表达于脑内。然而，其在中枢神经系统损伤中的表达和功能仍不清楚。本研究在成年大鼠中建立创伤性脑损伤（TBI）模型，研究 Numbl 在大脑皮质中的表达动态变化。Western blot 和免疫组织化学分析显示，Numbl 存在于正常脑内。它逐渐减少，在 TBI 后第 3 天达到最低点，然后在随后的几天内增加。双免疫荧光染色显示 Numbl 免疫反应性存在于神经元中，但不存在于星形胶质细胞和小胶质细胞中。此外，损伤后第 3 天是 caspase-3 变化所暗示的凋亡高峰。这些结果表明，Numbl 可能参与 TBI 的病理生理学过程，需要进一步研究以更好地了解其功能和机制。
