Pegram P S, DeChatelet L R, McCall C E
J Infect Dis. 1978 Nov;138(5):699-702. doi: 10.1093/infdis/138.5.699.
A study was undertaken to compare myeloperoxidase (MPO) activity in leukocytes from normal subjects and those with chronic granulomatous disease (CGD) and to eliminate further consideration of MPO as the oxidase responsible for the post-phagocytic respiratory burst and subsequent oxidase microbicidal sequence lacking in leukocytes from patients with CGD. With use of granule fractions isolated from a number of samples, MPO activity in several MPO-mediated biochemical systems (peroxidase assays, protein iodination, and amino acid decarboxylation) was measured. No difference was demonstrated between granule fraction preparations from normal subjects and those with CGD. These data show that MPO activity is normal in CGD leukocytes and are inconsistent with a role for MPO in initiating the post-phagocytic respiratory burst.
开展了一项研究，比较正常受试者与慢性肉芽肿病（CGD）患者白细胞中的髓过氧化物酶（MPO）活性，并进一步排除将MPO视为负责吞噬后呼吸爆发及后续氧化酶杀菌序列的氧化酶的可能性，CGD患者的白细胞缺乏该呼吸爆发及杀菌序列。利用从多个样本中分离出的颗粒组分，测定了几种MPO介导的生化系统（过氧化物酶测定、蛋白质碘化和氨基酸脱羧）中的MPO活性。正常受试者与CGD患者的颗粒组分制剂之间未显示出差异。这些数据表明，CGD白细胞中的MPO活性正常，这与MPO在引发吞噬后呼吸爆发中所起的作用不一致。
