Zu Mian, Zhou Dan, Gao Li, Liu Ai-lin, Du Guan-hua
Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Yao Xue Xue Bao. 2010 Mar;45(3):408-12.
To study in vitro anti-influenza viral activities of Chinese traditional patent medicines for influenza prevention and treatment, neuraminidase (NA) activity assay was used to examine NA inhibitory activity of 33 Chinese traditional patent medicines through fluorimetric assay, and influenza virus induced cytopathic effect (CPE) inhibition assay was used to verify their anti-influenza viral activities in vitro. The assay results showed that most liquid preparations displayed relatively high NA inhibitory activities, such as Shuanghuanglian oral liquid, Qingkailing oral liquid, Qingre Jiedu oral liquid, and Reduning injection. Among liquid preparations, Shuanghuanglian oral liquid not only displayed the highest NA inhibitory effect, but also exhibited obvious in vitro anti-viral activity in CPE experiment. Among solid preparations, Shuanghuanglian powder for injection showed the highest activity on NA inhibition, and Fufang Yuxingcao tablet showed relatively strong anti-influenza viral activity in CPE cells. From the results, it can be concluded that most Chinese traditional patent medicines possessed NA inhibitory activity, but only a few of them displayed significant in vitro anti-influenza viral activities. These results will provide important information for the isolation of active constituents, and for the clinical uses of Chinese traditional patent medicines for influenza treatment and prevention.
为研究用于预防和治疗流感的中成药的体外抗流感病毒活性，采用神经氨酸酶（NA）活性测定法，通过荧光测定法检测33种中成药的NA抑制活性，并采用流感病毒诱导的细胞病变效应（CPE）抑制试验来验证其体外抗流感病毒活性。试验结果表明，大多数液体制剂表现出较高的NA抑制活性，如双黄连口服液、清开灵口服液、清热解毒口服液和热毒宁注射液。在液体制剂中，双黄连口服液不仅表现出最高的NA抑制作用，而且在CPE实验中也表现出明显的体外抗病毒活性。在固体制剂中，注射用双黄连粉针剂对NA抑制活性最高，复方鱼腥草片在CPE细胞中表现出较强的抗流感病毒活性。从结果可以得出结论，大多数中成药具有NA抑制活性，但只有少数在体外表现出显著的抗流感病毒活性。这些结果将为活性成分的分离以及中成药在流感治疗和预防中的临床应用提供重要信息。
