Holmes S D, Dirmikis S M, Martin T J, Munro D S
J Endocrinol. 1978 Oct;79(1):121-30. doi: 10.1677/joe.0.0790121.
The activation of adenylate cyclase and the accumulation of cyclic AMP resulting from the action of human thyroid-stimulating hormone (TSH), long-acting thyroid stimulator (LATS) or LATS-protector (LATS-P) have been investigated in preparations of human thyroid membranes and slices. Human TSH significantly increased adenylate cyclase activity in membranes from non-toxic goitres whereas LATS and LATS-P had no consistent effect. However, pre-incubation of goitrous membranes with LATS--immunoglobulin G inhibited the effect of TSH on adenylate cyclase. When thyroid membranes were prepared from the glands of patients with Graves's disease neither TSH nor thyroid-stimulating immunoglobulins (TSIg) stimulated adenylate cyclase significantly. Whether from non-toxic goitres or thyrotoxic tissue, the concentration of TSH needed to induce half of the maximum response was lower in thyroid slices than in membranes. Both LATS and LATS-P significantly stimulated the accumulation of cyclic AMP in slices of goitrous tissue but thyrotoxic tissue slices did not respond. In goitrous slices, submaximum concentrations of TSH and TSIg caused additive responses in the accumulation of cyclic AMP but TSIg did not increase the maximum response to TSH.
在人甲状腺膜和切片制剂中,对人促甲状腺激素(TSH)、长效甲状腺刺激素(LATS)或LATS保护因子(LATS-P)作用导致的腺苷酸环化酶激活和环磷酸腺苷(cAMP)积累进行了研究。人TSH显著增加了来自非毒性甲状腺肿的膜中的腺苷酸环化酶活性,而LATS和LATS-P没有一致的作用。然而,用LATS-免疫球蛋白G对甲状腺肿膜进行预孵育可抑制TSH对腺苷酸环化酶的作用。当从格雷夫斯病患者的腺体制备甲状腺膜时,TSH和甲状腺刺激免疫球蛋白(TSIg)均未显著刺激腺苷酸环化酶。无论是来自非毒性甲状腺肿还是甲状腺毒症组织,诱导最大反应一半所需的TSH浓度在甲状腺切片中低于在膜中。LATS和LATS-P均显著刺激甲状腺肿组织切片中环磷酸腺苷的积累,但甲状腺毒症组织切片无反应。在甲状腺肿切片中,低于最大浓度的TSH和TSIg在环磷酸腺苷积累中引起相加反应,但TSIg并未增加对TSH的最大反应。