促甲状腺激素（TSH）和促甲状腺素受体抗体（TSAb）通过不同途径激活甲状腺腺苷酸环化酶。

TSH and thyroid stimulating antibodies (TSAb) activate thyroid adenylate cyclase through different pathways.

作者信息

Madsen S N, Bech K

出版信息

Acta Med Scand Suppl. 1979;624:35-42. doi: 10.1111/j.0954-6820.1979.tb00716.x.

DOI:10.1111/j.0954-6820.1979.tb00716.x

Abstract

Adenylate cyclase activity in human thyroid homogenates was studied after stimulation with thyrotropin (TSH) and thyroid stimulating antibodies (TSAb). The results show: 1) TSAb prepared from different patients with Graves' disease show different adenylate cyclase activation patterns and a lag phase is frequently observed. 2) TSH and TSAb appear to cause mutually inhibitory activation of thyroid adenylate cyclase. 3] The maximal adenylate cyclase activation is higher with TSH than with TSAb, but this could possibly be due to contamination of TSAb preparations with an adenylate cyclase inhibitor. 4) There is no absolute copurification of TSH sensitive and TSAb sensitive adenylate cyclase in various subcellular fractions of thyroid homogenate. 5) Incubation of thyroid homogenate with cortisol cause a dose dependent decrease in the adenylate cyclase response to TSAb whereas the response to TSH is either increased or unchanged. The results indicate that TSH and TSAb activate thyroid adenylate cyclase through different pathways in the plasma membrane.

摘要

在用促甲状腺激素（TSH）和甲状腺刺激抗体（TSAb）刺激后，对人甲状腺匀浆中的腺苷酸环化酶活性进行了研究。结果显示：1）从不同格雷夫斯病患者制备的TSAb表现出不同的腺苷酸环化酶激活模式，且经常观察到一个延迟期。2）TSH和TSAb似乎对甲状腺腺苷酸环化酶产生相互抑制性激活。3）TSH引起的腺苷酸环化酶最大激活高于TSAb，但这可能是由于TSAb制剂被腺苷酸环化酶抑制剂污染所致。4）在甲状腺匀浆的各种亚细胞组分中，TSH敏感性和TSAb敏感性腺苷酸环化酶不存在绝对的共纯化。5）甲状腺匀浆与皮质醇孵育会导致对TSAb的腺苷酸环化酶反应呈剂量依赖性降低，而对TSH的反应则要么增加要么不变。结果表明，TSH和TSAb通过质膜中的不同途径激活甲状腺腺苷酸环化酶。