Veterinary Neurology Center, College of Veterinary Medicine, Colorado State University, Fort Collins, CO, USA.
J Vet Intern Med. 2011 Mar-Apr;25(2):261-6. doi: 10.1111/j.1939-1676.2011.0692.x. Epub 2011 Feb 25.
Acute canine polyradiculoneuritis (ACP) is considered to be an animal model of the acute axonal form of Guillain-Barré syndrome (GBS) in humans. Various antecedent events have been associated with GBS, including bacterial or viral infection. The relationship between ACP and previous infection requires additional attention.
We hypothesized a relationship between ACP and serological evidence of exposure to Ehrlichia canis, Borrelia burgdorferi, Toxoplasma gondii, Neospora caninum, Campylobacter jejuni, and canine distemper virus (CDV).
Eighty-eight client-owned dogs, 44 with ACP, 44 age-matched controls.
Retrospective study with stored serum samples. Serum antibodies against the target organisms were measured with commercially available assays. Sera from dogs with and without ACP that were positive for T. gondii IgG by ELISA were assayed by an IgG heavy chain-specific, Western blot immunoassay.
Dogs with ACP (55.8%) were more likely to have T. gondii IgG serum antibody titers than dogs without ACP (11.4%). Serum antibodies from 8 affected dogs and 11 control dogs bound to T. gondii antigens with apparent molecular masses of 67, 61, 58, 45, 33, 24, 9, and 6 kDa. An antigen with an apparent molecular mass of 36 kDa was recognized by 2 dogs with ACP but none of the control dogs.
Results of this study suggest that ACP in some dogs, like GBS in some humans, may be triggered by T. gondii and a prospective study should be performed to further evaluate this potential association.
急性犬多发性神经病（ACP）被认为是人类格林-巴利综合征（GBS）急性轴索性形式的动物模型。各种前驱事件与 GBS 有关，包括细菌或病毒感染。ACP 与先前感染之间的关系需要进一步关注。
我们假设 ACP 与犬埃立克体、伯氏疏螺旋体、刚地弓形虫、新生隐球菌、空肠弯曲杆菌和犬瘟热病毒（CDV）血清学证据之间存在关联。
88 只患犬，44 只患有 ACP，44 只年龄匹配的对照组。
回顾性研究，使用储存的血清样本。使用商业上可获得的检测方法测量针对目标生物体的血清抗体。用 ELISA 检测到 T. gondii IgG 阳性的 ACP 犬和非 ACP 犬的血清，通过 IgG 重链特异性 Western blot 免疫测定进行检测。
患有 ACP 的犬（55.8%）比没有 ACP 的犬（11.4%）更有可能具有 T. gondii IgG 血清抗体滴度。8 只受影响的犬和 11 只对照犬的血清抗体与 T. gondii 抗原结合，其表观分子量分别为 67、61、58、45、33、24、9 和 6 kDa。ACP 犬中的一种抗原具有 36 kDa 的表观分子量，而 2 只 ACP 犬和 0 只对照犬的血清抗体与之结合。
本研究结果表明，某些犬的 ACP 可能像某些人类的 GBS 一样，由 T. gondii 引发，应该进行前瞻性研究以进一步评估这种潜在关联。
