Martinez-Anton L, Marenda M, Firestone S M, Bushell R N, Child G, Hamilton A I, Long S N, Le Chevoir M A R
Faculty of Veterinary & Agricultural Sciences, Department of Neurology & Neurosurgery, University of Melbourne, Melbourne, Vic., Australia.
Faculty of Veterinary & Agricultural Sciences, Department of Microbiology, University of Melbourne, Melbourne, Vic., Australia.
J Vet Intern Med. 2018 Jan;32(1):352-360. doi: 10.1111/jvim.15030. Epub 2018 Jan 22.
Acute polyradiculoneuritis (APN) is an immune-mediated peripheral nerve disorder in dogs that shares many similarities with Guillain-Barré syndrome (GBS) in humans, in which the bacterial pathogen Campylobacter spp. now is considered to be a major triggering agent. Little information is available concerning the relationship between APN and Campylobacter spp. in dogs.
HYPOTHESIS/OBJECTIVES: To estimate the association between Campylobacter spp. infection and APN. Associations with additional potential risk factors also were investigated, particularly consumption of raw chicken.
Twenty-seven client-owned dogs suffering from suspected APN and 47 healthy dogs, client-owned or owned by staff members.
Case-control study with incidence density-based sampling. Fecal samples were collected from each enrolled animal to perform direct culture, DNA extraction, and polymerase chain reaction (PCR) for detection of Campylobacter spp. In some cases, species identification was performed by sequence analysis of the amplicon. Data were obtained from the medical records and owner questionnaires in both groups.
In cases in which the fecal sample was collected within 7 days from onset of clinical signs, APN cases were 9.4 times more likely to be positive for Campylobacter spp compared to control dogs (P < 0.001). In addition, a significant association was detected between dogs affected by APN and the consumption of raw chicken (96% of APN cases; 26% of control dogs). The most common Campylobacter spp. identified was Campylobacter upsaliensis.
Raw chicken consumption is a risk factor in dogs for the development of APN, which potentially is mediated by infection with Campylobacter spp.
急性多神经根神经炎（APN）是犬类的一种免疫介导性周围神经疾病，与人类的吉兰 - 巴雷综合征（GBS）有许多相似之处，目前认为细菌病原体弯曲杆菌属是主要触发因素。关于犬类APN与弯曲杆菌属之间的关系，目前所知甚少。
假设/目的：评估弯曲杆菌属感染与APN之间的关联。还研究了与其他潜在风险因素的关联，特别是生鸡肉的摄入。
27只客户拥有的疑似APN犬和47只健康犬，包括客户拥有的犬以及工作人员拥有的犬。
采用基于发病密度抽样的病例对照研究。从每只纳入研究的动物采集粪便样本，进行直接培养、DNA提取和聚合酶链反应（PCR）以检测弯曲杆菌属。在某些情况下，通过扩增子的序列分析进行菌种鉴定。两组的数据均来自病历和主人问卷。
在临床症状出现后7天内采集粪便样本的病例中，APN病例弯曲杆菌属检测呈阳性的可能性是对照犬的9.4倍（P < 0.001）。此外，受APN影响的犬与生鸡肉摄入之间存在显著关联（APN病例的96%；对照犬的26%）。鉴定出的最常见弯曲杆菌属菌种是乌普萨拉弯曲杆菌。
生鸡肉摄入是犬类发生APN的一个风险因素，这可能是由弯曲杆菌属感染介导的。
