Department of General Genetics, Plant Molecular Biology and Biotechnology, University of Lodz, Banacha 12/16, Lodz, Poland.
Gene. 2011 Jul 1;480(1-2):21-7. doi: 10.1016/j.gene.2011.01.020. Epub 2011 Feb 22.
Although short interspersed elements (SINEs) were discovered nearly 30 years ago, the studies of these genomic repeats were mostly limited to animal genomes. Very little is known about SINEs in legumes--one of the most important plant families. Here we report identification, genomic distribution and molecular features of six novel SINE elements in Lotus japonicus (named LJ_SINE-1, -2, -3) and Medicago truncatula (MT_SINE-1, -2, -3), model species of legume. They possess all the structural features commonly found in short interspersed elements including RNA polymerase III promoter, polyA tail and flanking repeats. SINEs described here are present in low to moderate copy numbers from 150 to 3000. Bioinformatic analyses were used to searched public databases, we have shown that three of new SINE elements from M. truncatula seem to be characteristic of Medicago and Trifolium genera. Two SINE families have been found in L. japonicus and one is present in both M. truncatula and L. japonicus. In addition, we are discussing potential activities of the described elements.
尽管短散在元件（SINEs）在近 30 年前就被发现了，但这些基因组重复序列的研究大多局限于动物基因组。关于豆科植物（最重要的植物科之一）中的 SINEs 知之甚少。在这里，我们报告了在模式植物豌豆（命名为 Lj_SINE-1、-2、-3）和紫花苜蓿（MT_SINE-1、-2、-3）中 6 种新 SINE 元件的鉴定、基因组分布和分子特征。它们具有短散在元件中常见的所有结构特征，包括 RNA 聚合酶 III 启动子、多聚 A 尾和侧翼重复序列。这里描述的 SINEs 以 150 到 3000 个的低到中等拷贝数存在。通过生物信息学分析搜索公共数据库，我们已经表明，来自紫花苜蓿的三种新 SINE 元件似乎是 Medicago 和 Trifolium 属的特征。在豌豆中发现了两个 SINE 家族，一个存在于紫花苜蓿和豌豆中。此外，我们还在讨论所描述的元件的潜在活性。
