Department of Bioproducts and Biosystems Engineering, University of Minnesota, Center for Biorefining, 1390 Eckles Avenue, St Paul, MN 55108, USA.
Bioresour Technol. 2011 Apr;102(8):5138-44. doi: 10.1016/j.biortech.2011.01.091. Epub 2011 Feb 24.
The feasibility of growing Chlorella sp. in the centrate, a highly concentrated municipal wastewater stream generated from activated sludge thickening process, for simultaneous wastewater treatment and energy production was tested. The characteristics of algal growth, biodiesel production, wastewater nutrient removal and the viability of scale-up and the stability of continuous operation were examined. Two culture media, namely autoclaved centrate (AC) and raw centrate (RC) were used for comparison. The results showed that by the end of a 14-day batch culture, algae could remove ammonia, total nitrogen, total phosphorus, and chemical oxygen demand (COD) by 93.9%, 89.1%, 80.9%, and 90.8%, respectively from raw centrate, and the fatty acid methyl ester (FAME) content was 11.04% of dry biomass providing a biodiesel yield of 0.12 g-biodiesel/L-algae culture solution. The system could be successfully scaled up, and continuously operated at 50% daily harvesting rate, providing a net biomass productivity of 0.92 g-algae/(L day).
研究了在浓缩物（一种由活性污泥浓缩工艺产生的高浓度城市废水）中培养小球藻以同时进行废水处理和能源生产的可行性。考察了藻类生长、生物柴油生产、废水营养物去除以及放大规模的可行性和连续运行的稳定性。使用了两种培养基，即灭菌浓缩物（AC）和原浓缩物（RC）进行比较。结果表明，在 14 天的批式培养结束时，藻类可以分别从原浓缩物中去除氨、总氮、总磷和化学需氧量（COD）93.9%、89.1%、80.9%和 90.8%，其脂肪酸甲酯（FAME）含量为干生物质的 11.04%，提供的生物柴油产量为 0.12g-生物柴油/L-藻类培养液。该系统可以成功放大，并以 50%的每日收获率连续运行，提供 0.92g-藻类/（L-天）的净生物质生产力。
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