Department of Environmental Engineering, Technical University of Denmark, DK-2800 Kgs Lyngby, Denmark.
Bioresour Technol. 2011 Sep;102(18):8700-6. doi: 10.1016/j.biortech.2011.02.012. Epub 2011 Feb 24.
The present study investigated a two-stage anaerobic hydrogen and methane process for increasing bioenergy production from organic wastes. A two-stage process with hydraulic retention time (HRT) 3d for hydrogen reactor and 12d for methane reactor, obtained 11% higher energy compared to a single-stage methanogenic process (HRT 15 d) under organic loading rate (OLR) 3 gVS/(L d). The two-stage process was still stable when the OLR was increased to 4.5 gVS/(Ld), while the single-stage process failed. The study further revealed that by changing the HRT(hydrogen):HRT(methane) ratio of the two-stage process from 3:12 to 1:14, 6.7%, more energy could be obtained. Microbial community analysis indicated that the dominant bacterial species were different in the hydrogen reactors (Thermoanaerobacterium thermosaccharolyticum-like species) and methane reactors (Clostridium thermocellum-like species). The changes of substrates and HRT did not change the dominant species. The archaeal community structures in methane reactors were similar both in single- and two- stage reactors, with acetoclastic methanogens Methanosarcina acetivorans-like organisms as the dominant species.
本研究探索了一种两段式厌氧产氢和甲烷工艺，以提高有机废物的生物能源产量。与单级产甲烷工艺（水力停留时间 15 天，有机负荷率 3 gVS/（L d）相比，在有机负荷率 3 gVS/（L d）下，采用水力停留时间 3 天的两段式产氢反应器和 12 天的甲烷反应器的两段式工艺可获得 11%的更高能量。当有机负荷率增加到 4.5 gVS/（Ld）时，两段式工艺仍然稳定，而单级工艺则失败。研究进一步表明，通过将两段式工艺的 HRT（产氢）：HRT（产甲烷）从 3:12 改为 1:14，可获得 6.7%的更多能量。微生物群落分析表明，在产氢反应器（Thermoanaerobacterium thermosaccharolyticum 样物种）和甲烷反应器（Clostridium thermocellum 样物种）中，优势细菌种类不同。底物和 HRT 的变化并没有改变优势物种。在单级和两级反应器中，甲烷反应器中的古菌群落结构相似，以产乙酸甲烷菌 Methanosarcina acetivorans 样生物为主要物种。
