Falasca C A, Gili M, Grana D, Gomez E, Zoppi J, Mareso E
Instituto Latinoamericano de Investigaciones Medicas Universidad del Salvador (ILAIMUS), Buenos Aires, Argentina.
Rev Inst Med Trop Sao Paulo. 1990 May-Jun;32(3):151-61. doi: 10.1590/s0036-46651990000300002.
Eighteen Cebus apella monkeys, (juvenile and adult of both sexes) were inoculated five years ago, with three Trypanosoma cruzi strains (CA1, n = 10; Colombian, n = 4 and Tulahuen, n = 4), either by conjunctival or intraperitoneal route, once or repeatedly. Parasitological, hematological, serological, enzymatic, radiographic, electro and echocardiographic findings have been previously published and they are similar to those observed in human pathology. The most frequent electrocardiographic alteration was right branch bundle block. Six animals, chosen at random, were sacrificed. Those sacrificed 20 to 25 months post-first inoculation showed focal accumuli of leukocytes with myocytolysis. Foci of diffuse interstitial fibrosis with mild infiltrate of leukocytes among fibers were observed in the animals sacrificed 36 to 47 months post-inoculation. No parasites were seen. The lesions were more prominent in the ventricular walls and the septum. The fact that the infiltrates were predominant in the animals sacrificed at a shorter time after first inoculation and that fibrosis was more severe in those sacrificed at a longer time suggests that there is a progression of the infiltrative lesions to fibrosis, with a leukocytic activity indicative of a chronic phase. These lesions are similar to those described in human chronic Chagas' disease. This would demonstrate that this model is useful in evaluating a progress in the knowledge of the pathogenesis which is still a controversial issue, immunology, immunogenesis and chemotherapeutic agents of the chronic and indeterminate phases of this disease.
18只僧帽猴（雌雄幼猴及成年猴）于5年前通过结膜或腹腔途径，单次或反复接种了3种克氏锥虫菌株（CA1，n = 10；哥伦比亚株，n = 4；图拉韦恩株，n = 4）。寄生虫学、血液学、血清学、酶学、放射学、电学及超声心动图检查结果此前已发表，且与人类病理学中观察到的结果相似。最常见的心电图改变是右束支传导阻滞。随机挑选6只动物进行处死。首次接种后20至25个月处死的动物显示有白细胞灶性聚集伴肌细胞溶解。接种后36至47个月处死的动物中观察到弥漫性间质纤维化灶，纤维间有轻度白细胞浸润。未发现寄生虫。病变在心室壁和室间隔更为明显。首次接种后较短时间处死的动物中浸润更为显著，而较长时间处死的动物中纤维化更为严重，这一事实表明浸润性病变向纤维化发展，且白细胞活性提示为慢性期。这些病变与人类慢性恰加斯病中描述的病变相似。这将证明该模型在评估该疾病慢性和不确定期的发病机制、免疫学、免疫发生及化疗药物等方面的知识进展方面是有用的，而这些仍是有争议的问题。
