Gambari R, Barbieri R, Nastruzzi C, Feriotto G, Piva R, Natali P G, Giacomini P
Institute of Chemical Biology, University of Ferrara, Italy.
J Biol Regul Homeost Agents. 1990 Jul-Sep;4(3):98-106.
The binding of nuclear proteins from human tumor cells to synthetic double stranded oligonucleotides mimicking upstream regions of the HLA-DR alpha gene was studied. As the HLA-DR alpha gene is inducible by interferon(IFN)-gamma, nuclear extracts were also purified from IFN-gamma treated cells. Our data indicate that a) nuclear binding proteins (named IFN-gamma-B3 and Z-B1/B2) are detectable, specific for the IFN-gamma and Z boxes of the human HLA-DR alpha gene; b) both IFN-gamma-B3 and Z-B1/B2 are present in HLA-D negative cell lines and c) the content of IFN-gamma-B3 and Z-B1/B2 is not modulated by IFN-gamma treatment. These data suggest that the presence in the nuclear compartment of these factors, presumably necessary for the correct regulation of the HLA-DR alpha gene, is not sufficient to explain its differential constitutive and induced expression in a variety of in vitro cultured cell lines of different lineage.
研究了来自人类肿瘤细胞的核蛋白与模拟HLA - DRα基因上游区域的合成双链寡核苷酸的结合情况。由于HLA - DRα基因可被干扰素（IFN）-γ诱导，还从经IFN - γ处理的细胞中纯化了核提取物。我们的数据表明：a）可检测到核结合蛋白（命名为IFN - γ - B3和Z - B1/B2），它们对人类HLA - DRα基因的IFN - γ和Z框具有特异性；b）IFN - γ - B3和Z - B1/B2都存在于HLA - D阴性细胞系中；c）IFN - γ - B3和Z - B1/B2的含量不受IFN - γ处理的调节。这些数据表明，这些因子存在于核区室中，推测它们对于HLA - DRα基因的正确调控是必需的，但这不足以解释其在不同谱系的各种体外培养细胞系中的差异组成性表达和诱导表达。
