Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, Guangzhou Road 300, Nanjing 210029, China.
Toxicol Lett. 2011 May 10;202(3):253-60. doi: 10.1016/j.toxlet.2011.02.011. Epub 2011 Feb 24.
Iodinated contrast media (CM) can induce apoptosis and necrosis of renal tubular cells. The injuries of endothelial cells induced by CM on the systemic condition have not been fully understood. To assess the toxic effects of non-ionic CM on the glomerular and aortic endothelial cells, iopromide and iodixanol, two kinds of representative non-ionic CM, were used for the in vivo study. Sixty aged rats were respectively received the agents or normal sodium intravascularly. No obvious apoptosis and morphological change was detected in the glomerular and aortic endothelial cells apart from renal tubules after CM administration. However, expressions of the nitric oxide synthase (eNOS) in glomerular endothelium were decreased at 12h after CM injection. Furthermore, plasma creatinine and endothelin-1 were increased and plasma nitric oxide (NO) was decreased significantly after CM administration. However, we failed to observe the significant increase of plasma von Willebrand Factor. These results suggest that non-ionic iodinated CM do not induce apoptosis and necrosis of glomerular and aortic endothelial cells in vivo. Decreased eNOS expression and increased plasma endothelin-1 may be involved in non-ionic iodinated CM-induced endothelial dysfunction and kidney injury.
碘造影剂（CM）可诱导肾小管细胞凋亡和坏死。CM 对全身状况下内皮细胞的损伤尚未完全了解。为了评估非离子型 CM 对肾小球和主动脉内皮细胞的毒性作用，使用两种代表性的非离子型 CM，碘普罗胺和碘克沙醇，进行了体内研究。60 只老年大鼠分别接受了这些药物或正常的静脉内钠。除了肾小管外，CM 给药后肾小球和主动脉内皮细胞没有明显的凋亡和形态变化。然而，CM 注射后 12 小时，肾小球内皮细胞中一氧化氮合酶（eNOS）的表达减少。此外，CM 给药后血浆肌酐和内皮素-1显著增加，血浆一氧化氮（NO）显著降低。然而，我们未能观察到血浆血管性血友病因子的显著增加。这些结果表明，非离子型碘造影剂在体内不会诱导肾小球和主动脉内皮细胞的凋亡和坏死。eNOS 表达减少和血浆内皮素-1增加可能参与非离子型碘造影剂诱导的内皮功能障碍和肾损伤。
