Department of Advanced Medicine in Biotechnology and Robotics, Graduate School of Medicine, Nagoya University, Higashi-ku, Nagoya 461-0047, Japan.
Biomaterials. 2011 May;32(15):3729-38. doi: 10.1016/j.biomaterials.2011.01.013. Epub 2011 Feb 26.
Induced pluripotent stem (iPS) cells are expected to provide a source of tissue, a renewable cell source for tissue engineering, and a method for in vitro drug screening for patient-specific or disease-specific treatment. A simple technology by which iPS cells can be differentiated effectively and in large quantities is strongly desired. In this paper, a new device (Tapered Soft Stencil for Cluster Culture: TASCL) is proposed for the easy and efficient formation of EBs which can be used in regenerative medicine. This device was compared with the two major methods currently being evaluated, namely the HD method and the Terasaki® plate (MWC substitution), in terms of the efficiency, morphology and acquired number of EB formation. Using the TASCL device, the shape of the EBs formed was almost a perfect sphere, and the formation was also faster than for the two other methods. There was little variability in the number of cells. Moreover, EBs formed using the TASCL device had the ability to differentiate into all three germ layers, and differentiation of EBs from the TASCL culture into hepatic cells was confirmed. In conclusion, it appears that the TASCL device can be utilized for EB formation to generate cells for regenerative medicine applications.
诱导多能干细胞（iPS 细胞）有望为组织工程提供组织来源、可再生的细胞来源，以及用于针对患者或疾病特异性治疗的体外药物筛选的方法。强烈需要一种能够有效且大量分化 iPS 细胞的简单技术。在本文中，提出了一种新的设备（用于集落培养的锥形软模板：TASCL），用于易于且高效地形成可用于再生医学的 EBs。该设备在效率、形态和获得的 EB 形成数量方面与目前正在评估的两种主要方法（HD 方法和 Terasaki®板（MWC 替代））进行了比较。使用 TASCL 设备形成的 EBs 的形状几乎为完美的球体，形成速度也比其他两种方法快。细胞数量的变化很小。此外，使用 TASCL 设备形成的 EBs 具有分化为三个胚层的能力，并且已经证实 TASCL 培养物中的 EBs 可以分化为肝细胞。总之，TASCL 设备似乎可用于 EB 形成，以产生用于再生医学应用的细胞。
