Jiang Mengjun, Zhou Yaoyuan, Yang Min, Zhang Rongjun, Zou Meifen, Cai Gangming, Pan Donghui
Jiangsu Institute of Nuclear Medicine, Key Laboratory of Nuclear Medicine, Ministry of Health, Wuxi 214063, China.
Zhongguo Zhong Yao Za Zhi. 2010 Nov;35(22):3056-9.
To investigate the effect of betulinic acid (BA) on the proliferation, migration, apoptosis and cell cycle of pancreatic cancer cells (BxPC-3) in vitro and elucidate the underlying.
The effect of BA on the proliferation of BxPC-3 was measured by using sulforhodamine B (SRB) assay. Migratory ability of BxPC3 cells were detected by wound healing assay, and the morphological change was observed with light microscope. The influence of BA on cell cycle of BxPC-3 cells was tested by flow cytometry (FCM). Apoptosis was analyzed by using Hochest33342-PI double staining. Western blot technologies were applied to detect the expression of Bcl-2 and Bax.
BA exhibited significant cell proliferation and migration inhibition, as well as its potency of inducing apoptosis in BxPC-3 cells in vitro in a dose-dependent manner. The IC50 value for 72 h was 16.54 mg x L(-1). Cell migration was significantly inhibited at 5 mg x L(-1) of BA. Cells treated with BA showed increased cell population in G0 phase, with decreased G2/M phase population. The expression of Bax and Bcl-2 was up and down-regulated respectively in BA-treated BxPC-3 cells in a dose-dependent manner.
BA exerted potent effect on growth inhibition, G0 cell cycle arrest and induction of apoptosis in BxPC-3 cells in vitro, possibly associated with the down-regulation of Bcl-2 and up-regulation of Bax expression. The potent antitumor capacity of BA suggested that it could be a promising new anticancer agent in human pancreatic cancer treatment.
研究桦木酸（BA）对体外培养的胰腺癌细胞（BxPC-3）增殖、迁移、凋亡及细胞周期的影响，并阐明其潜在机制。
采用磺酰罗丹明B（SRB）法检测BA对BxPC-3细胞增殖的影响。通过划痕实验检测BxPC-3细胞的迁移能力，并用光学显微镜观察形态变化。采用流式细胞术（FCM）检测BA对BxPC-3细胞周期的影响。利用Hochest33342-PI双染法分析细胞凋亡情况。应用蛋白质免疫印迹技术检测Bcl-2和Bax的表达。
BA在体外对BxPC-3细胞具有显著的增殖和迁移抑制作用，且诱导凋亡的能力呈剂量依赖性。72小时的半数抑制浓度（IC50）值为16.54mg·L⁻¹。5mg·L⁻¹的BA可显著抑制细胞迁移。经BA处理的细胞在G0期细胞比例增加，G2/M期细胞比例减少。在经BA处理的BxPC-3细胞中，Bax和Bcl-2的表达分别呈剂量依赖性上调和下调。
BA在体外对BxPC-3细胞具有强大的生长抑制、G0期细胞周期阻滞及诱导凋亡作用，可能与下调Bcl-2表达和上调Bax表达有关。BA强大的抗肿瘤能力表明它可能是一种有前景的新型抗胰腺癌药物。
