Transfecting neural crest cells in avian embryos using in ovo electroporation.
作者信息
Kadison Stephanie R, Krull Catherine E
机构信息
Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, USA.
出版信息
CSH Protoc. 2008 Jan 1;2008:pdb.prot4925. doi: 10.1101/pdb.prot4925.
PMID:21356679
Abstract
INTRODUCTIONThis protocol outlines a method for introducing genes into the neural crest cells of avian embryos at vagal/cardiac and trunk levels. Electroporation offers the unique opportunity to misexpress genes or "knock down" the expression of their protein products using short hairpin RNAs (shRNAs) within targeted populations of cells. Electroporated cells can be visualized by using DNA plasmids that contain enhanced green fluorescent protein (EGFP) or other fluorescent reporter proteins.
摘要
Zotero 插件