Misquitta Leonie, Wei Qin, Paterson Bruce M
CSH Protoc. 2008 Feb 1;2008:pdb.prot4917. doi: 10.1101/pdb.prot4917.
INTRODUCTIONRNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a method for collection of Drosophila embryos for RNA interference (RNAi) experiments. The embryos are collected in a simple, homemade apparatus, arrayed on prepared glass slides, and readied for injection. It is important to keep the embryos moist and oxygenated. Work expeditiously, because embryos should be injected within 30-60 min after collection.
引言
RNA干扰（RNAi）是确定特定基因在果蝇胚胎发育过程中作用的一种强大方法。本方案描述了一种用于RNA干扰（RNAi）实验的果蝇胚胎收集方法。胚胎在一个简单的自制装置中收集，排列在准备好的载玻片上，然后准备进行注射。保持胚胎湿润并使其获得氧气很重要。要迅速操作，因为胚胎应在收集后30 - 60分钟内进行注射。
