Weigel Detlef, Glazebrook Jane
CSH Protoc. 2008 Feb 1;2008:pdb.prot4941. doi: 10.1101/pdb.prot4941.
INTRODUCTIONThis protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.
引言
本方案描述了用于切片以及后续免疫组织化学或原位杂交的植物组织的制备方法。在切片之前,将感兴趣的组织固定并包埋在石蜡中。