Fischer Andrew H, Jacobson Kenneth A, Rose Jack, Zeller Rolf
CSH Protoc. 2008 May 1;2008:pdb.prot4987. doi: 10.1101/pdb.prot4987.
INTRODUCTIONThis protocol describes the sectioning of tissues embedded in paraffin blocks. Paraffin sections require extensive fixation and processing steps but provide superior morphology compared with other sectioning methods. Sectioning paraffin blocks requires experience and should be learned from an experienced researcher, if possible.
引言
本方案描述了石蜡包埋组织的切片制作方法。石蜡切片需要经过广泛的固定和处理步骤,但与其他切片方法相比,能提供更优的形态结构。石蜡块切片需要经验,如果可能的话,应向有经验的研究人员学习。