通过抑制性消减杂交鉴定差异基因：I. 消减cDNA或基因组DNA文库的制备。
Identification of differential genes by suppression subtractive hybridization: I. Preparation of subtracted cDNA or genomic DNA library.
作者信息
Rebrikov Denis V
出版信息
CSH Protoc. 2008 Jul 1;2008:pdb.prot4855. doi: 10.1101/pdb.prot4855.
INTRODUCTIONSuppression subtractive hybridization (SSH) is one of the most powerful and popular methods for generating subtracted cDNA or genomic DNA libraries. This technique can be used to compare two mRNA populations and obtain cDNAs representing genes that are either overexpressed or exclusively expressed in one population as compared to another. It can also be used for comparison of genomic DNA populations. This protocol describes the preparation of a subtracted cDNA or genomic DNA library, and includes methods for cDNA synthesis, tester and driver DNA digestion, and adapter ligation.
引言
抑制性消减杂交（SSH）是用于构建消减cDNA或基因组DNA文库的最强大且最常用的方法之一。该技术可用于比较两个mRNA群体，并获得代表与另一个群体相比在一个群体中过表达或特异性表达的基因的cDNA。它也可用于比较基因组DNA群体。本方案描述了消减cDNA或基因组DNA文库的制备方法，包括cDNA合成、测试者和驱动者DNA消化以及接头连接的方法。
Zotero 插件