斑马鱼胚胎的全胚胎RNA原位杂交：探针合成
Whole mount RNA in situ hybridization on zebrafish embryos: probe synthesis.
作者信息
Moens Cecilia
机构信息
Division of Basic Science, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
出版信息
CSH Protoc. 2008 Aug 1;2008:pdb.prot5036. doi: 10.1101/pdb.prot5036.
PMID:21356893
Abstract
INTRODUCTIONThis protocol describes methods for synthesizing digoxigenin- and fluorescein-labeled RNA probes from linearized plasmids or PCR products for use in whole-mount RNA in situ hybridization on zebrafish embryos.
摘要
引言
本方案描述了从线性化质粒或PCR产物合成地高辛和荧光素标记的RNA探针的方法，用于斑马鱼胚胎的全胚胎原位杂交。
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