在无饲养层条件下培养人类胚胎干细胞。

Culturing human embryonic stem cells in feeder-free conditions.

作者信息

McElroy Sohyun L, Reijo Pera Renee A

机构信息

Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, CA 94304-5542, USA.

出版信息

CSH Protoc. 2008 Sep 1;2008:pdb.prot5044. doi: 10.1101/pdb.prot5044.

DOI:10.1101/pdb.prot5044

PMID:21356913

Abstract

INTRODUCTIONHuman embryonic stem cells (hESCs) have the potential to differentiate into all three germ layers and proliferate in long-term culture in vitro. hESCs can provide a cell source for the testing of novel therapies, drug screening, and functional genomics applications. Undifferentiated hESCs can be maintained and proliferated on mouse embryonic fibroblasts (MEFs) or human feeder cells. In this protocol, we describe the culture of hESCs in feeder-free conditions on Matrigel with MEF-conditioned medium. This protocol can be used for applications such as genetic modification of hESCs without feeder cell contamination.

摘要

引言

人类胚胎干细胞（hESCs）有潜力分化为所有三个胚层，并能在体外长期培养中增殖。hESCs可为新型疗法测试、药物筛选及功能基因组学应用提供细胞来源。未分化的hESCs可在小鼠胚胎成纤维细胞（MEFs）或人饲养层细胞上维持和增殖。在本方案中，我们描述了在无饲养层条件下，使用MEF条件培养基在基质胶上培养hESCs的方法。该方案可用于诸如对hESCs进行基因改造而无饲养层细胞污染的应用。