在活体系统中以单转录本分辨率成像实时基因表达：基于ImageJ分析的单个mRNA粒子追踪
Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis.
作者信息
Wells Amber L, Condeelis John S, Singer Robert H, Zenklusen Daniel
机构信息
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
出版信息
CSH Protoc. 2007 Nov 1;2007:pdb.prot4872. doi: 10.1101/pdb.prot4872.
Abstract
INTRODUCTIONThis protocol describes the use of ImageJ software (freely available from NIH) to analyze particle dynamics in a cell using time-lapse movie frames or image stacks of fluorescent mRNA particles. Maximum intensity projections and kymographs are produced.
摘要
引言
本方案描述了使用ImageJ软件（可从美国国立卫生研究院免费获取），通过延时电影帧或荧光mRNA颗粒的图像堆栈来分析细胞中颗粒动力学。生成最大强度投影图和动态光密度图。
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